p 30: effect of fasudil on acrylamide-induced cytotoxicity in pc12 cells through evaluation of ros and mtt test

introduction: acrylamide (acr) known as a neurotoxic agent in human and animals. previous studies have been shown; fasudil improves neurological deficit and neuronal damage. in this study, the effect of fasudil, potent rho-kinase inhibitor, on acr-induced cytotoxicity was evaluated using pc12 cells as a suitable in vitro model. materials and methods: pc12 cells were exposed to different concentrations of fasudil (5,10, 25, 50 and 100 µm) for 24 h. then, acr 6 mm was added. after 24 h exposure with acr, cell viability was determined using mtt test. for evaluation reactive oxygen species production, 2, 7-dichlorofluorescein diacetate (dcfh-da) method was used. in addition the levels of bax and bcl-2 proteins were evaluated using western blot analysis. results: acr exposure increased ros production and bax/bcl-2 ratio while decreased cell viability. pretreatment with fasudil (50 µm) for 24 h inhibited ros production (*** p< 0.001) and increased viability (*** p< 0.001). also, fasudil could decrease bax/bcl-2 ratio but was not significantly. conclusion: the oxidative stress and apoptosis pathway play important roles in acr toxicity on pc12 cells. fasudil exhibited protective effects on acr toxicity through inhibition of oxidative stress and apoptosis pathway.